上海儒百生物(Schbio Biotech)
专业血液免疫学产品生产商

Product Description
Biotinylated HumanFas / TNFRSF6 / CD95, Avitag-His  Protein
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Brand : RecProtein®
Synonym : FAS,ALPS1A,APO1,APT1,CD95,FAS1,FASTM,TNFRSF6,FasR
Molecule : FAS
Catalog : B3274
Qty : 1mg, 5mg
18521561575  需求咨询
Datasheet

Source

Human Fas, His Tag Avitag  is expressed from human 293 cells (HEK293).

Predicted N-terminus: Gln 26


Molecular Characterization

The protein has a calculated MW of 18.2 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.


Endotoxin

Less than 0.01 EU/mg by the LAL method.


Purity

>95% as determined by SDS-PAGE.


Bioactivity

ELISA

BIACORE (With our Research Grade Biosimilar Antibody products);


Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.


Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.


Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

4-8°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.


Background

The Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). is a death receptor on the surface of cells that leads to programmed cell death (apoptosis). It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.


Reference

(1) Wajant H, 2002, Science 296 (5573): 1635–6.

(2) Huang B, et al., 1996, Nature 384 (6610): 638–41.

(3) Chen L, et al., 2010, Nature 465 (7297): 492–6.



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