Source

Human CD68, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Asn 22 - Ser 319 (Accession # AAH15557).

Predicted N-terminus: Asn 22

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

Endotoxin

Less than 0.01 EU/mg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Bioactivity

ELISA ；

BIACORE (With our Research Grade Biosimilar Antibody products);

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

4-8°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

Background

CD antigen CD68 is also known as Macrosialin, Gp110, which belongs to the LAMP family. CD68 / Macrosialin is highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 / Macrosialin could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 / Macrosialin binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Reference

(1) Kunisch E., et al., 2004, Ann. Rheum. Dis. 63:774-784.

(2) Beranek J.T., 2005, Ann. Rheum. Dis. 64:342-343.

(3) Gottfried E., et al., 2008, Scand. J. Immunol. 67:453-463.