Source

Mouse CD155, Mouse IgG2a Fc Tag is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Leu 348 (Accession # Q8K094-1).

Predicted N-terminus: Asp 29

Molecular Characterization

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 61.8 kDa. The protein migrates as 35 kDa and 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU/μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Bioactivity

ELISA ；

BIACORE (With our Research Grade Biosimilar Antibody products);

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

4-8°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

Background

CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.'

Reference

(1) Zhang P., et al., 2008, Proc. Natl. Acad. Sci., 105 (47): 18284-18289.

(2) Maier MK., et al., 2007, European Journal of Immunology, 37 (8): 2214-2225.

(3) He Y., et al., 2000, Proc. Natl. Acad. Sci., 97 (1): 79-84.