Source

Human B7-H4, mouse Fc Tag, is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # Q7Z7D3-1).

Predicted N-terminus: Phe 29

Molecular Characterization

This protein carries a mouse IgG Fc tag at the C-terminus.

Endotoxin

Less than 0.01 EU/μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Bioactivity

ELISA ；

BIACORE (With our Research Grade Biosimilar Antibody products);

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

4-8°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

Background

V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

Reference

(1) Salceda S., et al., 2005, Exp. Cell Res. 306:128-141.

(2) Kryczek I., et al., 2006, J. Exp. Med. 203:871-881.

(3) Miyatake T., et al., 2007, Gynecol. Oncol. 106:119-127.