上海儒百生物(Schbio Biotech)
专业血液免疫学产品生产商

Product Description
Biotinylated Human PD-L1 / B7-H1 , His Tag
在线客服
Brand : RecProtein®
Synonym : PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
Molecule : PD-L1
Catalog : B3568
Qty : 1mg, 5mg
18521561575  需求咨询
Datasheet

Source

Biotinylated Human PD-L1   (H-PDL1-7)   is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

Predicted N-terminus: Phe 19


Molecular Characterization

This protein carries an Avi tagat the C-terminus, followed by a polyhistidine tag.



Endotoxin

Less than 0.01 EU/mg by the LAL method.


Purity

>95% as determined by SDS-PAGE.


Bioactivity

ELISA

BIACORE (With our Research Grade Biosimilar Antibody products);


Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.


Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.


Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

4-8°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.


Background

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.


Reference

(1) Dong H., et al., 1999, Nat. Med. 5:1365-1369.

(2) Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.

(3) Nishimura H., et al., 2001, Trends in Immunology 22:265.


会员登录
登录
其他帐号登录:
留言
回到顶部