Product Description

Mononuclear cells (MNCs) are separated from umbilical cord blood by means of a density gradient centrifugation protocol. The mononuclear cells are composed of lymphocytes (T cells, B cells, and NK cells), monocytes, dendritic cells, and stem/progenitor cells. These cells are critical components of the immune system and play key roles in the humoral and cell mediated immune responses. Mononuclear cells are commonly used in research and clinical applications in the areas of immunology, infectious disease, hematological malignancies, vaccine development, transplant therapy, and high-throughput screening.

StemExpress offers highly pure and viable mononuclear cells from cord blood in an assortment of pre-selected volumes and formats (cryopreserved or fresh). This highly pure population of cells makes them ideal for further isolations of specific cell types within the mononuclear fraction.

Cord blood is collected from mothers that are negative for HIV, HepB, and HepC during pregnancy. Testing on cord blood can be provided as a custom order.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Format

Catalog #

QTY/Unit

Cryopreserved

CBMNC150C

CBMNC200C

CBMNC300C

CBMNC015C

CBMNC025C

150 million/vial

200 million/vial

300 million/vial

15 million/vial

50 million/vial

Product Specification

Species:	Human
Cell Source:	Umbilical Cord Blood
Product Type:	Primary Cells
Cell Types:	Mononuclear Cells, Monocytes, Lymphocytes, Stem/Progenitor Cells, Dendritic Cells
Donor Attributes:	Maternal Blood HIV-, HepB-, HepC-
Single or Mixed Donor:	Single
Viability:	≥85% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Contains:	Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)