Peripheral Blood CD4+CD45RA+ Naïve Helper T Cells

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Catalog PB445RA005C
Qty 5million/vial
15044661636
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Naïve helper T cells are mature T cells that have left the thymus and have yet to be exposed to an antigen presented by MHC class II molecules of an antigen-presenting cell (APC). In humans, CD4+ naïve helper T cells are constantly recirculating into the secondary lymphoid tissues in order to optimize the opportunity of finding their specific antigen.


Human peripheral blood CD4+CD45RA+ naïve helper T cells are enriched by depleting cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, CD45RO, TCRγ/δ, and CD235a from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+CD45RA+ naïve helper T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.



Format
Catalog #
QTY/Unit
Cryopreserved
PB445RA005C
5 million/vial



Product Specification


Species:
Human
Cell Source:
Peripheral Blood
Product Type:
Primary Cells
Cell Type:
T Cells
Donor Attributes:
HIV-, HepB-, HepC-
Purity:
≥85% by Flow Cytometry
Viability:
≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Contains:
Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)


Flow Data


Figure 1. Representative flow data of the enriched CD4+CD45RA+ cells (91.0%) from peripheral blood prior to cryopreservation.