Peripheral Blood CD4+CD45RO+ Memory T Cells

Catalog PB445RO005C
Qty 5million/vial
18521561575
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Peripheral blood CD4+CD45RO+ memory T cells are formed after a naïve T cell has come into contact with an antigen. Memory T cells remain inactive until they encounter the same antigen and reactivate to form effector T cells. Having encountered the antigen previously this second encounter produces a faster and stronger immune response to eliminate the foreign invader or cancer cell.

Human CD4+CD45RO+ memory T cells are isolated from peripheral blood mononuclear cells by depleting cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD45RA, CD56, CD123, TCRγ/δ, and CD235a are from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+CD45RO+ memory T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

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Format
Catalog #
QTY/Unit
Cryopreserved
PB445RO005C
5 million/vial



Product Specification


Species:
Human
Cell Source:
Peripheral Blood
Product Type:
Primary Cells
Cell Type:
T Cells
Donor Attributes:
HIV-, HepB-, HepC-
Purity:
≥85% by Flow Cytometry
Viability:
≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Contains:
Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)


Flow Data

Figure 1. Representative flow data of enriched CD4+CD45RO+ memory T cells from peripheral blood prior to cryopreservation.