Peripheral Blood CD4+CD45RO+ Memory T Cells

Catalog PB445RO005C
Qty 5million/vial

Peripheral blood CD4+CD45RO+ memory T cells are formed after a naïve T cell has come into contact with an antigen. Memory T cells remain inactive until they encounter the same antigen and reactivate to form effector T cells. Having encountered the antigen previously this second encounter produces a faster and stronger immune response to eliminate the foreign invader or cancer cell.

Human CD4+CD45RO+ memory T cells are isolated from peripheral blood mononuclear cells by depleting cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD45RA, CD56, CD123, TCRγ/δ, and CD235a are from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+CD45RO+ memory T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Custom OrdersWe work closely with our customers to provide them with the product they need. For a custom order, please call and speak to one of our sales specialists at 530-303-3828.

Catalog #
5 million/vial

Product Specification

Cell Source:
Peripheral Blood
Product Type:
Primary Cells
Cell Type:
T Cells
Donor Attributes:
HIV-, HepB-, HepC-
≥85% by Flow Cytometry
≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)

Flow Data

Figure 1. Representative flow data of enriched CD4+CD45RO+ memory T cells from peripheral blood prior to cryopreservation.