专业血液免疫学产品供应商

Product Description
Peripheral Blood CD4+CD25+ Regulatory T Cells
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Brand : StemExpress®
Molecule : 人外周血
Catalog : PB425002C
Qty : 2 million/vial
18521561575  需求咨询
Datasheet

Human peripheral blood regulatory T cells (Tregs) are a subpopulation of T cells that play an important role in maintaining self-tolerance in the adaptive immune system by suppressing potentially deleterious activities of CD4+ helper T cells, which, if not regulated, can cause a variety of autoimmune disorders.

Peripheral Blood CD4+CD25+ Tregs are enriched in a two-step process. First, cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a are depleted from the peripheral blood mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+ T cells. Then, enriched CD4+ T cells are selected for CD25+ cells by positive selection using antiCD25 immunomagnetic particles allowing for the enrichment of CD4+CD25+ regulatory T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.


Format
Catalog #
QTY/Unit
Cryopreserved
PB425002C
2 million/vial



Product Specification


Species:
Human
Cell Source:
Peripheral Blood
Product Type:
Primary Cells
Cell Type:
T Cells
Donor Attributes:
HIV-, HepB-, HepC-
Purity:
≥85% by Flow Cytometry
Viability:
≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Contains:
Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)


Flow Data

Figure 1. Representative flow data of peripheral blood enriched CD4+CD25+ Tregs prior to cryopreservation.


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