Peripheral Blood CD4+CD25+ Regulatory T Cells

Human peripheral blood regulatory T cells (Tregs) are a subpopulation of T cells that play an important role in maintaining self-tolerance in the adaptive immune system by suppressing potentially deleterious activities of CD4⁺ helper T cells, which, if not regulated, can cause a variety of autoimmune disorders.

Peripheral Blood CD4⁺CD25⁺ Tregs are enriched in a two-step process. First, cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a are depleted from the peripheral blood mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4⁺ T cells. Then, enriched CD4⁺ T cells are selected for CD25⁺ cells by positive selection using antiCD25 immunomagnetic particles allowing for the enrichment of CD4⁺CD25⁺ regulatory T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Product Specification

Flow Data

Figure 1. Representative flow data of peripheral blood enriched CD4⁺CD25⁺ Tregs prior to cryopreservation.