Schbio Biotech

Product Description
PB Macrophage
在线客服
Brand : StemExpress®
Molecule : Peripheral Blood
Catalog : PBMAC001.5C
Qty : 1.5 million/vial
Price : Require
Datasheet

Upon tissue damage or infection, monocytes leave the bloodstream and are rapidly recruited to the tissue, where they differentiate into tissue macrophages. Part of the innate immunity, macrophages are specialized cells that recognize, engulf and destroy target cells or pathogens. They are remarkably plastic and can change their functional phenotype depending on the various microbes or invaders they encounter. Along with their ability to clear pathogens, macrophages can also instruct other immune cells to take part in the immune response by acting as an antigen-presenting cell (APC).

The isolation of mature macrophages from tissue can be difficult. Therefore the use of monocyte derived macrophages (MDM) for functional studies is a very attractive alternative. Non monocytes are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD14+ monocytes. The untouched monocytes are cultured for 4~5 days with 10% FBS in the presence of MCSF and IL4. After culture, cells are checked for the expression of CD11b, CD18, CD68, and HLA-DR by flow cytometry.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.


Format
Catalog #
QTY/Unit
Cryopreserved
PBMAC001.5C
1.5 million/vial


Product Specification


Species:
Human
Cell Source:
Peripheral Blood
Product Type:
Cultured Cells
Cell Type:
Macrophage
Donor Attributes:
HIV-, HepB-, HepC-
Purity:
≥90% by Flow Cytometry
Viability:
≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Contains:
Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)



会员登录
登录
留言
回到顶部