Dendritic cells (DCs) form a critical interface between innate and adaptive immunity. DCs continuously sample their environment for antigens by means of endocytosis. Referred to as an antigen presenting cell (APC), DCs capture, process and present these antigens to naïve T-helper cells to initiate an immune response. There is great heterogeneity in DC phenotypes due to their stage of differentiation or according to their response and interaction with cytokines and/or growth factors in their microenvironment. This can make isolating a specific subset of DCs challenging. Additionally, each subset is rare in number, compounding the difficulty in isolating these cells. Culturing of CD14⁺ monocytes in the presence of specific growth factors and cytokines can induce differentiation into immature DCs (iDC), known as monocyte derived immature dendritic cells (MoDCs). Cultured immature dendritic cells remove the phenotype heterogeneity issue as well as the rarity of these cells.

Human peripheral blood monocyte-derived immature dendritic cells are derived from negatively selected monocytes. Non-monocytes are labeled and depleted from the peripheral blood mononuclear cell population using immunomagnetic particles leaving purified, untouched monocytes. Untouched monocytes are cultured for 4~5 days with 10% FBS in the presence of GM-CSF and IL4. After culture, cells are checked for the expression of CD11c, HLA DR, and CD83 and the lack of CD14 expression by flow cytometry.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Format	Catalog #	QTY/Unit
Cryopreserved	PBMAC001.5C	1.5 million/vial

Product Specification

Species:	Human
Cell Source:	Peripheral Blood
Product Type:	Cultured Cells
Cell Type:	Dendritic Cells
Donor Attributes:	HIV-, HepB-, HepC-
Purity:	≥90% CD11c and MHCII <30% CD83≤5% CD14
Viability:	≥85% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Contains:	Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)