Peripheral blood CD19+CD27- naïve B cells are B cells that have not encountered an antigen. Upon antigen contact naïve B cells will undergo isotype class-switch recombination and somatic hypermutation and develop into plasma and memory B cells.

Human CD19⁺CD27^–naïve B cells are isolated from peripheral blood mononuclear cells by means of negative selection. Cells expressing CD2, CD3, CD14, CD16, CD27, CD36, CD43, CD56, CD66b, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD19⁺CD27^– naïve B cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Format	Catalog #	QTY/Unit
Cryopreserved	PB1927N01C PB1927N02C	1 million/vial 2 million/vial

Product Specification

Species:	Human
Cell Source:	Peripheral Blood
Product Type:	Primary Cells
Cell Type:	B Cells
Donor Attributes:	HIV-, HepB- HepC-
Purity:	≥85% by Flow Cytometry
Viability:	≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Contains:	Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)

Flow Data

Figure 1. Representative flow data of peripheral blood enriched CD19⁺CD27^– naive B cells prior to cryopreservation.