Peripheral Blood CD8+ T Cells

Human peripheral blood CD8⁺ cytotoxic T cells, also known as cytotoxic T lymphocytes, defend the body against intracellular pathogens, such as viruses and bacteria, as well as cancerous cells. CD8⁺ T cells distinguish infected or diseased cells from healthy cells by recognizing antigens presented by MHC class I molecules located on the surface of the inflicted cells. Once the CD8 T cells recognize a specific antigen it releases cytotoxins that result in apoptosis of the infected or diseased cell.

Human CD8⁺ cytotoxic T cells are isolated from peripheral blood mononuclear cells by depleting cells expressing CD4, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a using immunomagnetic particles leaving purified, untouched CD8+ cytotoxic T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Product Specification

Flow Data

Figure 1. Representative histogram of peripheral blood enriched CD8⁺ cytotoxic T cells (93.72%).