Human peripheral blood CD8+ cytotoxic T cells, also known as cytotoxic T lymphocytes, defend the body against intracellular pathogens, such as viruses and bacteria, as well as cancerous cells. CD8+ T cells distinguish infected or diseased cells from healthy cells by recognizing antigens presented by MHC class I molecules located on the surface of the inflicted cells. Once the CD8 T cells recognize a specific antigen it releases cytotoxins that result in apoptosis of the infected or diseased cell.
Human CD8+ cytotoxic T cells are isolated from peripheral blood mononuclear cells by depleting cells expressing CD4, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a using immunomagnetic particles leaving purified, untouched CD8+ cytotoxic T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Format | Catalog # | QTY/Unit |
Cryopreserved | PB08010C | 10 million/vial |
Product Specification
Species: | Human |
Cell Source: | Peripheral Blood |
Product Type: | Primary Cells |
Cell Type: | T Cells |
Donor Attributes: | HIV-, HepB-, HepC- |
Purity: | ≥85% by Flow Cytometry |
Viability: | ≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw. |
Contains: | Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO) |
Flow Data
Figure 1. Representative histogram of peripheral blood enriched CD8+ cytotoxic T cells (93.72%).