Human T cells are lymphocytes that are generally characterized by the expression of CD3 on the cell surface. These cells originate in the bone marrow, migrate to the thymus to undergo maturation, and are one of the key cell types involved in cell mediated immunity. T cells are involved in destroying invading organisms and also aid in the activation of B cells through the thymus dependent response. There are several different subsets of T cells, including helper T cells (naïve and memory), cytotoxic T cells (naïve and memory), regulatory T cells (Tregs) and natural killer T (NKT) cells.
Human CD3+ pan T cells are selected from peripheral blood mononuclear cells by means of negative selection. Cells expressing CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD3+ pan T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population. Positively selected CD3+ T cells are available as a custom order.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
HIV-, HepB-, HepC-
≥85% by Flow Cytometry
≥95% by Flow CytometryNote: For cryopreserved samples the freeze thaw cycle may decrease cell viability by 10-15% post thaw.
Fresh: PBS with 5% FBS and 0.5% BSA Frozen: CryoStor™ CS10 (10% DMSO)
Figure 1. Representative histogram of enriched CD3+ pan T cells from peripheral blood prior to cryopreservation.