Peripheral Blood CD19+ B Cells

Human B cells are lymphocytes that are characterized by the expression of CD19 on the cell surface. These cells are one of the main cell types involved in the humoral immune response. Upon activation, due to an encounter with an antigen, B cells will act as antigen presenting cells (APCs), make antibodies against foreign antigens, and develop into memory B cells or plasma cells.

Human CD19⁺ B cells are isolated from peripheral blood mononuclear cells by means of negative selection. Cells expressing CD2, CD3, CD14, CD16, CD36, CD43, CD56, CD66b, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD19⁺ B cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population. Positively selected CD19⁺ B cells are available as a custom order.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Product Specification

Flow Data

Figure 1. Representative histogram of enriched CD19⁺ B cells from peripheral blood prior to cryopreservation.