纯度

> 95 % as determined by SDS-PAGE

内毒素

< 0.01 EU per μg of the protein asdetermined by the LAL method

表达宿主

HEK293 Cells

种属

Mouse

缓冲液

Lyophilized from sterile 50mM Tris, 100mMNaCl, pH 8.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added asprotectants before lyophilization. Specific concentrations are included in thehardcopy of COA.

运输方式

In general, recombinant proteins areprovided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They areshipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve monthsfrom date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that theprotein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitutioninstruction is sent along with the products. Please refer to it for detailedinformation.

Alternate Names for Recombinant Mouse TIM-4 Protein

FLJ27515
Smuckler
T-cell immunoglobulin and mucin domain containing 4
T-cell immunoglobulin and mucin domain-containing protein 4
T-cell membrane protein 4
TIM4
TIM-4
TIMD4
TIMD-4

Background

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The mouse TIM-4 cDNA encodes a 343 amino acid (aa) precursor that includes a 22 aa signal sequence, a 257 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1, -2, or -3. Within the ECD, mouse TIM-4 shares 27 - 33% aa sequence identity with mouse TIM-1, -2, and -3. The ECD of mouse TIM-4 shares 45% aa sequence identity with that of human and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction ofTIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.