纯度

> 95 % as determined by SDS-PAGE

内毒素

< 0.01 EU per μg of the protein asdetermined by the LAL method

蛋白构建

A DNA sequence encoding the mature form of mouse TIM2 extracellular domain was fused with Fc region of mouse IgG at the N-terminus.

表达宿主

HEK293 Cells

种属

Mouse

缓冲液

Lyophilized from sterile 50mM Tris, 100mMNaCl, pH 8.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added asprotectants before lyophilization. Specific concentrations are included in thehardcopy of COA.

运输方式

In general, recombinant proteins areprovided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They areshipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve monthsfrom date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that theprotein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hard copy of COA with reconstitutioninstruction is sent along with the products. Please refer to it for detailed information.

Alternate Names for Recombinant Mouse TIM-2 Protein

T Cell immunoglobulin and mucin domain protein 2
TIM2
TIM-2

Background

T cell immunoglobulin and mucin domain‑2 (TIM‑2) is an approximately 50 kDa member of the TIM family of immune regulatory proteins. TIMs are type I transmembrane proteins with one Ig‑like V domain and a Ser/Thr‑rich mucin domain in their extracellular regions (1). Mature mouse TIM‑2 consists of a 210 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic domain (2). Rat TIM‑2, by contrast, possesses the same structural architecture as mouse but contains an additional 46 aa within the mucin domain. Over comparable regions of the ECD, mouse and rat TIM‑2 share 74% aa sequence identity. The isolated ECD forms dimers and tetramers in solution, and these structures suggest the possibility of TIM‑2/TIM‑2 cis interactions (3). TIM‑2 is expressed on Th2 cells, B cells, oligodendrocytes, hepatocytes, hepatic stellate cells, and epithelial cells lining the bile duct and renal tubules (4 ‑ 9). It is upregulated on Th2 cells during cellular activation and functions as a negative regulator of Th2 cell expansion and immune responses (8 ‑ 11). TIM‑2 binds to ligands expressed on activated B cells, macrophages, dendritic cells, and T cells (8, 12). Its ligation by Semaphorin 4A induces tyrosine phosphorylation in the cytoplasmic domain of TIM‑2 (12). TIM‑2 binding to ligand(s) on hepatocytes inhibits hepatocyte differentiation and proliferation (7). TIM‑2 additionally plays a role in iron homeostasis by serving as an endocytic receptor for the heavy chain of Ferritin (H‑Ferritin) (4, 6).

Rodriguez-Manzanet, R. et al. (2009) Immunol. Rev. 229:259.
McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
Santiago, C. et al. (2007) Immunity 26:299.
Todorich, B. et al. (2008) J. Neurochem. 107:1495.
Ruddell, R.G. et al. (2009) Hepatology 49:887.