纯度

> 95 % as determined by SDS-PAGE.

内毒素

< 0.01 EU per mg protein as determinedby the LAL method.

蛋白构建

A DNA sequence encoding the human NCR3LG1 (NP_001189368.1)(Met1-Ser262) was expressed with the Fc region of human IgG1 at the C-terminus.

NPNo.

NP_001189368.1

表达宿主

HEK293 Cells

预测 N 端

Asp 25

分子量

The recombinant human NCR3LG1 consists 476amino acids and predicts a molecular mass of 53.4 kDa.

种属

Human

缓冲液

Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added asprotectants before lyophilization. Specific concentrations are included in thehardcopy of COA.

运输方式

In general, recombinant proteins areprovided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They areshipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve monthsfrom date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that theprotein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitutioninstruction is sent along with the products. Please refer to it for detailedinformation.

Background

The B7 family ofgenes is essential in the regulation of the adaptive immune system. one ofwhich is the recently discovered B7H6. Humans and rats have a single B7H6 gene;however, many B7H6 genes were detected in a single large cluster in the Xenopusgenome. \rChimeric antigen receptor (CAR) T-cell therapies have demonstrateddurable and potentially curative therapeutic efficacy against B-cell leukemiain clinical trials. In this study, B7H6, a ligand for the NK cell activatingreceptor NKp30, was targeted to create a CAR that targets multiple tumor types.B7H6 is expressed on various primary human tumors, including leukemia, lymphomaand gastrointestinal stromal tumors, but it is not constitutively expressed onnormal tissues.

Reference

(1) Wu MR, et all.,2015, Gene Ther.22(8):675-84.

(2) Phillips M, etall., 2016, Biopolymers. 106(5):658-72.

(3) Flajnik MF, etall., 2012 Immunogenetics. 64(8):571-90.