纯度

> 95 % as determined by SDS-PAGE.

内毒素

< 0.01 EU per mg protein as determinedby the LAL method.

蛋白构建

A DNA sequence encoding the human PDCD1(NP_005009.2) (Met1-Gln167) was expressed with the Fc region of mouse IgG1 atthe C-terminus.

NPNo.

NP_005009.2

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Leu 25

分子量

The recombinant human PDCD1 consists of 377amino acids and predicts a molecular mass of 42.3 kDa.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added asprotectants before lyophilization. Specific concentrations are included in thehardcopy of COA.

运输方式

In general, recombinant proteins areprovided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They areshipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve monthsfrom date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that theprotein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitutioninstruction is sent along with the products. Please refer to it for detailedinformation.

PD-1 Protein, Human, Recombinant (FcTag): Alternative Names

CD279 Protein, Human; hPD-1 Protein, Human;hPD-l Protein, Human; hSLE1 Protein, Human; PD-1 Protein, Human; PD1 Protein,Human; SLEB2 Protein, Human

PD-1 Background Information

Programmed cell death 1, also known asPDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptorbelonging to the CD28/CTLA-4 family negatively regulates antigen receptorsignaling by recruiting protein tyrosine phosphatase, SHP-2 upon interactingwith either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cellproliferation and production of related cytokines including IL-1, IL-4, IL-1and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.In addition, coligation of PD1 inhibits BCR-mediating signal bydephosphorylating key signal transducer. PD1 has been suggested to be involvedin lymphocyte clonal selection and peripheral tolerance, and thus contributesto the prevention of autoimmune diseases. Furthermore, PD1 is shown to be aregulator of virus-specific CD8+ T cell survival in HIV infection. As a cellsurface molecule, PDCD1 regulates the adaptive immune response. Engagement ofPD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cellproliferation, cytokine production, and cytolytic function.